4JDE
Crystal structure of PUD-1/PUD-2 heterodimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.99985 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.991, 89.775, 102.503 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.900 |
| R-factor | 0.1934 |
| Rwork | 0.192 |
| R-free | 0.22530 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.212 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 25.000 | 25.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 5.140 | 1.900 |
| Rmerge | 0.073 | 0.037 | 0.446 |
| Number of reflections | 39660 | ||
| <I/σ(I)> | 13.3 | ||
| Completeness [%] | 98.9 | 97.6 | 88.6 |
| Redundancy | 4.4 | 4.4 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | 0.1M HEPES (pH 7.2), 4.3M NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
