4JCY

Crystal structure of the Restriction-Modification Controller Protein C.Csp231I OR operator complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	120
Detector technology	PIXEL
Collection date	2012-06-05
Detector	PSI PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 61
Unit cell lengths	62.340, 62.340, 158.080
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	53.990 - 1.800
R-factor	0.14788
Rwork	0.147
R-free	0.16260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lfp
RMSD bond length	0.004
RMSD bond angle	0.952
Data reduction software	MOSFLM
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.990	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.059	0.551
Number of reflections	30476
<I/σ(I)>	12.8	2.6
Completeness [%]	99.5	99.8
Redundancy	4.2	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	Protein was dialysed against the buffer containing 0.1 M NaCl, 50 mM TRIS-HCl pH 8.0, 1 mM EDTA. Crystallisation conditions: 0.2 M sodium iodide, 0.1 M Bis-Tris Propane pH 6.0, 15 % (w/v) PEG3350, 10 mM spermidine, protein dimer/DNA molar ratio 1:1, protein concentration 4.6 mg/ml , VAPOR DIFFUSION, HANGING DROP, temperature 289K