4JCV

Crystal structure of the RecOR complex in an open conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2011-04-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	P 1
Unit cell lengths	63.130, 93.110, 92.350
Unit cell angles	103.60, 110.42, 106.22

Refinement procedure

Resolution	83.070 - 3.340
R-factor	0.24203
Rwork	0.240
R-free	0.27810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vdd 1w3s
RMSD bond length	0.010
RMSD bond angle	1.329
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.6.0116)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	83.070	3.520
High resolution limit [Å]	3.340	3.340
Rmerge		0.061
Number of reflections	24029
<I/σ(I)>		8.8
Completeness [%]	94.2
Redundancy	1.8	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	296	0.1M MES pH 6.5, 10% dioxane, 1.6M ammonium sulphate, VAPOR DIFFUSION, HANGING DROP, temperature 296K