4JCT
ClpP2 from Listeria monocytogenes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-03-25 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 120.110, 148.460, 103.470 |
| Unit cell angles | 90.00, 119.31, 90.00 |
Refinement procedure
| Resolution | 14.980 - 2.600 |
| R-factor | 0.21259 |
| Rwork | 0.211 |
| R-free | 0.24833 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3v5e |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.996 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.080 | 0.615 |
| Number of reflections | 46079 | |
| <I/σ(I)> | 10.3 | 2 |
| Completeness [%] | 94.6 | 92.2 |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.1 M Mes, 1.0 M LiCl, 10% PEG6000 , pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
