4JCP
Structure of Cyclophilin B from Brugia malayi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.13 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 57.830, 57.830, 97.100 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.810 - 1.650 |
| R-factor | 0.159 |
| Rwork | 0.158 |
| R-free | 0.18750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dyw |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.289 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.5.2) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 19.810 | 1.690 | |
| High resolution limit [Å] | 1.650 | 7.380 | 1.650 |
| Rmerge | 0.073 | 0.028 | 0.404 |
| Number of reflections | 23092 | 300 | 1549 |
| <I/σ(I)> | 21.81 | 52.8 | 4.32 |
| Completeness [%] | 99.2 | 93.2 | 91.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 298 | 23.4 mg/ml BrmaA.01375.b, 3.15M ammonium sulfate, 100mM sodium citrate pH 5, direct cryoprotection. , VAPOR DIFFUSION, SITTING DROP, temperature 298K |
