4JCO
1.7 A resolution structure of wild type malate dehydrogenase from haloarcula marismortui
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-25 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 126.813, 114.065, 124.022 |
| Unit cell angles | 90.00, 93.49, 90.00 |
Refinement procedure
| Resolution | 42.964 - 1.700 |
| R-factor | 0.2257 |
| Rwork | 0.224 |
| R-free | 0.25700 |
| Structure solution method | FOURIER, DIFFERENCE FOURIER |
| Starting model (for MR) | 2j5k |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.175 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 123.792 | 1.740 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.109 | 1.224 |
| Number of reflections | 192220 | |
| <I/σ(I)> | 8.52 | 1.1 |
| Completeness [%] | 99.6 | 99.8 |
| Redundancy | 3.79 | 3.79 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 58% MPD, pH 7.00, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
