4JCN
Structure of ESP, serine protease from Staphylococcus epidermidis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-04-15 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.410, 60.360, 42.340 |
| Unit cell angles | 90.00, 98.59, 90.00 |
Refinement procedure
| Resolution | 41.860 - 1.800 |
| R-factor | 0.17473 |
| Rwork | 0.173 |
| R-free | 0.19859 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qy6 |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.051 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.870 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.039 | 0.201 |
| Number of reflections | 17810 | |
| <I/σ(I)> | 20.2 | 5.9 |
| Completeness [%] | 97.4 | 95.1 |
| Redundancy | 5.16 | 5.14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 20% PEG 8000, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
