4JC8
Crystal Structure of HOPS component Vps33 from Chaetomium thermophilum
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-08-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97930, 0.9640 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.936, 64.436, 151.685 |
| Unit cell angles | 90.00, 91.77, 90.00 |
Refinement procedure
| Resolution | 35.951 - 2.600 |
| R-factor | 0.1877 |
| Rwork | 0.185 |
| R-free | 0.24690 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.218 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | PHENIX (dev_1144) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 43028 | |
| <I/σ(I)> | 15.1 | 2.6 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 12% (w/v) PEG 3350, 10 mM barium chloride, pH 8.5, vapor diffusion, sitting drop, temperature 298K |
