4JBR
tRNA-guanine transglycosylase Y330C mutant as covalently linked dimer in space group P6(5)22
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-02-15 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.91841 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 127.552, 127.552, 116.270 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.111 - 2.920 |
R-factor | 0.2053 |
Rwork | 0.203 |
R-free | 0.24550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1pud |
RMSD bond length | 0.003 |
RMSD bond angle | 0.665 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.970 |
High resolution limit [Å] | 2.920 | 2.920 |
Number of reflections | 12637 | |
<I/σ(I)> | 6.06 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 15 | 16.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | MgAc 0.2M, PEG3350 20%; 2% DMSO and 10% glycerol in cryoprotection buffer, VAPOR DIFFUSION, SITTING DROP, temperature 293K |