4JAH
Crystal structure of 2-Selenouridine containing RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-07-29 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9795 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 46.444, 46.444, 120.612 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.220 - 1.500 |
R-factor | 0.20975 |
Rwork | 0.208 |
R-free | 0.23807 |
Structure solution method | 246D |
RMSD bond length | 0.014 |
RMSD bond angle | 2.305 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.222 | 1.550 |
High resolution limit [Å] | 1.500 | 1.500 |
Number of reflections | 24253 | |
<I/σ(I)> | 20.5 | 2.4 |
Completeness [%] | 97.3 | 64.6 |
Redundancy | 4.1 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 10% v/v MPD, 40 mM NaCacodylate pH 7.0, 12 mM Spermine tetra-HCl, 80 mM Sodium Chloride, 12mM Potassium Chloride, 20 mM Magnesium Chloride , VAPOR DIFFUSION, HANGING DROP, temperature 298K |