4JAA
Factor inhibiting HIF-1 alpha in complex with consensus ankyrin repeat domain-(d)LEU peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-04-23 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.91730 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 86.079, 86.079, 147.029 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.800 - 2.390 |
R-factor | 0.1873 |
Rwork | 0.184 |
R-free | 0.21890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1h2k |
RMSD bond length | 0.010 |
RMSD bond angle | 1.142 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (0.1.16) |
Phasing software | PHASER (2.3.0) |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.804 | 2.550 |
High resolution limit [Å] | 2.390 | 2.390 |
Rmerge | 0.089 | 0.508 |
Number of reflections | 22451 | |
<I/σ(I)> | 9.3 | 2.6 |
Completeness [%] | 99.3 | 98.7 |
Redundancy | 5 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 1.6M AMMONIUM SULPHATE, 6% PEG 400, 0.1M HEPES PH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |