4J9Y

Calcium-calmodulin complexed with the calmodulin binding domain from a small conductance potassium channel splice variant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 12.3.1
Synchrotron site	ALS
Beamline	12.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-12-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.12
Spacegroup name	C 1 2 1
Unit cell lengths	77.630, 66.820, 64.830
Unit cell angles	90.00, 94.48, 90.00

Refinement procedure

Resolution	38.879 - 1.510
R-factor	0.1844
Rwork	0.183
R-free	0.21630
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.289
Data scaling software	SCALA (3.3.20)
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	38.879	38.879	1.550
High resolution limit [Å]	1.510	6.750	1.510
Rmerge		0.018	0.682
Total number of observations		2215	19146
Number of reflections	49769
<I/σ(I)>	24.1	36.1	1.1
Completeness [%]	96.3	78.2	94
Redundancy	5.3	4.7	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	298	1.25 M lithium sulfate, 0.5 M ammonium sulfate, 0.1 M sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K