4J9V

Crystal Structure of the TrkA Gating ring bound to ATP-gamma-S

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-09-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.99997
Spacegroup name	P 61 2 2
Unit cell lengths	109.324, 109.324, 337.656
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.170 - 3.051
R-factor	0.2144
Rwork	0.211
R-free	0.27200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.021
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.100
High resolution limit [Å]	3.050	8.270	3.050
Rmerge	0.103	0.036	0.889
Number of reflections	23765
<I/σ(I)>	8.6
Completeness [%]	100.0	100	100
Redundancy	11.7	10.1	12.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	298	30% PEG 400, 100 mM Glycine, 200 mM MgSO4, pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K