4J9S
Human DNA polymerase eta-DNA translocated binary complex: with TA base pair
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2010-01-05 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 61 |
Unit cell lengths | 98.820, 98.820, 81.110 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.527 - 1.950 |
R-factor | 0.1863 |
Rwork | 0.183 |
R-free | 0.22220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3mr2 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.173 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.250 | 2.000 |
High resolution limit [Å] | 1.950 | 2.180 | 1.950 |
Rmerge | 0.067 | 0.258 | 0.643 |
Number of reflections | 32266 | ||
<I/σ(I)> | 17.95 | 8.32 | 3.51 |
Completeness [%] | 98.1 | 98.6 | 99.4 |
Redundancy | 7.1 | 7.11 | 7.03 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | PEG2K-MME, KCl, MES, pH 6.5, vapor diffusion, hanging drop, temperature 298K |