4J9R
Human DNA polymerase eta-DNA translocated binary complex with TG mispair
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2010-01-05 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 61 |
Unit cell lengths | 98.820, 98.820, 81.110 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 26.911 - 2.350 |
R-factor | 0.2012 |
Rwork | 0.197 |
R-free | 0.24770 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3mr2 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.278 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.710 | 2.410 |
High resolution limit [Å] | 2.350 | 2.630 | 2.350 |
Rmerge | 0.102 | 0.424 | 0.754 |
Number of reflections | 18824 | ||
<I/σ(I)> | 17.27 | 5.04 | 2.8 |
Completeness [%] | 99.8 | 99.9 | 99.6 |
Redundancy | 5.1 | 5.12 | 5.08 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | PEG2K-MME, KCl, MES, pH 6.5, vapor diffusion, hanging drop, temperature 298K |