4J9N
Human DNA polymerase eta-DNA ternary complex: misincorporation G opposite T after a G at the primer 3' end (GA/G)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2011-08-05 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 61 |
Unit cell lengths | 98.664, 98.664, 81.960 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.482 - 1.956 |
R-factor | 0.1663 |
Rwork | 0.165 |
R-free | 0.19860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3mr2 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.229 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.150 | 1.980 |
High resolution limit [Å] | 1.950 | 2.100 | 1.950 |
Rmerge | 0.093 | 0.256 | 0.426 |
Number of reflections | 32381 | ||
<I/σ(I)> | 19.2 | 8.16 | 4.82 |
Completeness [%] | 98.6 | 99.7 | 100 |
Redundancy | 6.4 | 6.3 | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | PEG2K-MME, KCl, MES, pH 6.5, vapor diffusion, hanging drop, temperature 298K |