4J94
Crystal structure of MycP1 from the ESX-1 type VII secretion system
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08B1-1 |
Synchrotron site | CLSI |
Beamline | 08B1-1 |
Temperature [K] | 77 |
Detector technology | CCD |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97895 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.490, 77.290, 84.930 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.218 - 1.857 |
R-factor | 0.1571 |
Rwork | 0.155 |
R-free | 0.19130 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.106 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.20) |
Phasing software | PHASER |
Refinement software | PHENIX (dev_1284) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.040 | 47.040 | 2.370 |
High resolution limit [Å] | 1.581 | 7.120 | 2.250 |
Rmerge | 0.042 | 0.206 | |
Total number of observations | 3485 | 15874 | |
Number of reflections | 27671 | ||
<I/σ(I)> | 13.4 | 13.5 | 3.6 |
Completeness [%] | 99.9 | 98.6 | 100 |
Redundancy | 5 | 4.6 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.77 | 293 | 0.1M Bis-Tris propane 0.18 M sodium thiocyanate 26% PEG 3350, pH 6.77, VAPOR DIFFUSION, SITTING DROP, temperature 293K |