4J91
Diamond-shaped octameric structure of KtrA with ADP bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-06-19 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.933400 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 147.932, 84.630, 113.675 |
Unit cell angles | 90.00, 128.37, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 89.123 | 42.315 | 2.930 |
High resolution limit [Å] | 2.781 | 8.800 | 2.780 |
Rmerge | 0.018 | 0.827 | |
Rmeas | 0.021 | 0.925 | |
Rpim | 0.010 | 0.410 | |
Total number of observations | 4044 | 19307 | |
Number of reflections | 27727 | ||
<I/σ(I)> | 21.6 | 71.4 | 2.3 |
Completeness [%] | 99.7 | 97.6 | 99.1 |
Redundancy | 4.9 | 4.5 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 0.1M citrate pH5.6, 14.5% polyethylene glycol 2000, 0.2M ammonium sulphate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |