4J8Z

Crystal Structure of the Human SPOP BTB Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-02-01
Detector	PSI PILATUS 6M
Wavelength(s)	0.91730
Spacegroup name	C 1 2 1
Unit cell lengths	111.390, 71.580, 62.220
Unit cell angles	90.00, 117.21, 90.00

Refinement procedure

Resolution	58.020 - 2.420
R-factor	0.2091
Rwork	0.207
R-free	0.25490
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.583
Data reduction software	XDS
Data scaling software	SCALA (0.1.27)
Phasing software	PHASER (2.5.2)
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	58.020
High resolution limit [Å]	2.420
Rmerge	0.044
Number of reflections	16513
<I/σ(I)>	17.2
Completeness [%]	98.9
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	32% PEG3350, 17% isopropanol, 0.1M tris-HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K