4J8L

CRYSTAL STRUCTURE OF THE PYRIDOXAL-5'-PHOSPHATE DEPENDENT protein YhfS from Escherichia coli

Experimental procedure

Experimental method	SAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-02-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 62 2 2
Unit cell lengths	134.915, 134.915, 75.142
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.121 - 1.651
R-factor	0.1705
Rwork	0.169
R-free	0.19520
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.068
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall
Low resolution limit [Å]	46.121
High resolution limit [Å]	1.651
Number of reflections	48578
Completeness [%]	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	1.4M sodium acetate, 0.1M sodium cacodylate , pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K