4J8E

Middle domain of Hsc70-interacting protein, crystal form I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2010-10-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00497
Spacegroup name	P 43 21 2
Unit cell lengths	79.856, 79.856, 97.311
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.600
R-factor	0.187
Rwork	0.184
R-free	0.24810
Structure solution method	SIRAS
RMSD bond length	0.013
RMSD bond angle	1.363
Data scaling software	SCALA (3.2.25)
Phasing software	SHELX
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	96.135	96.135	2.840
High resolution limit [Å]	2.600	8.510	2.600
Rmerge	0.080	0.031	0.609
Total number of observations		1478	4647
Number of reflections	9666
<I/σ(I)>	15.4	18	1.2
Completeness [%]	97.5	98.2	85.6
Redundancy	5.1	4.4	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	2.3 Na-malonate, pH 6.0, vapor diffusion, hanging drop, temperature 291K