4J8C
Crystal structure of the dimerization domain of Hsc70-interacting protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-26 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.90004 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 28.775, 43.978, 32.211 |
| Unit cell angles | 90.00, 93.44, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.100 |
| R-factor | 0.1363 |
| Rwork | 0.135 |
| R-free | 0.16180 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.722 |
| Data scaling software | SCALA (3.2.25) |
| Phasing software | SHELX |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 43.980 | 43.980 | 1.160 |
| High resolution limit [Å] | 1.100 | 3.470 | 1.100 |
| Rmerge | 0.057 | 0.037 | 0.323 |
| Total number of observations | 2402 | 16236 | |
| Number of reflections | 32315 | ||
| <I/σ(I)> | 13.4 | 8.8 | 2 |
| Completeness [%] | 98.6 | 71.2 | 97.5 |
| Redundancy | 3.6 | 3.1 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 2.52 Na-malonate, pH 7.0, vapor diffusion, hanging drop, temperature 291K |
