4J7Z
Thermus thermophilus DNAJ J- and G/F-DOMAINS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2006-02-01 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 31 |
| Unit cell lengths | 85.990, 85.990, 72.720 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.240 - 1.640 |
| R-factor | 0.216 |
| Rwork | 0.214 |
| R-free | 0.24600 |
| Structure solution method | RIPAS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.174 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.700 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Rmerge | 0.066 | 0.353 |
| Number of reflections | 72046 | |
| <I/σ(I)> | 12.5 | 2.8 |
| Completeness [%] | 97.7 | 94.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | PEG1500 15%, 0.1 M STRONTIUM CHLORIDE , VAPOR DIFFUSION, HANGING DROP |
