4J7Q
Crystal structure of Saccharomyces cerevisiae Sfh3 complexed with phosphatidylinositol
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 4A |
Synchrotron site | PAL/PLS |
Beamline | 4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-11-17 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97949 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 52.889, 144.149, 55.656 |
Unit cell angles | 90.00, 111.28, 90.00 |
Refinement procedure
Resolution | 34.400 - 1.550 |
R-factor | 0.221 |
Rwork | 0.221 |
R-free | 0.23900 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.580 |
High resolution limit [Å] | 1.550 | 1.550 |
<I/σ(I)> | 32.4 | 4.3 |
Completeness [%] | 99.1 | 98.8 |
Redundancy | 2.8 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | 0.1M MES, 20% PEG 8000, 0.2M ammonium sulfate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |