4J7O

Structure of the N-terminal Repeat Domain of Rickettsia Sca2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-10-21
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9769
Spacegroup name	P 21 21 2
Unit cell lengths	82.020, 102.870, 55.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	64.000 - 2.175
R-factor	0.1829
Rwork	0.179
R-free	0.22710
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.718
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXS
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall
Low resolution limit [Å]	64.000
High resolution limit [Å]	2.175
Rmerge	0.045
Number of reflections	24526
<I/σ(I)>	13.5
Completeness [%]	97.4
Redundancy	6.83

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	0.2M sodium citrate, 18-20% (w/v) PEG 3350 , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K