4J6Y
Hfq from Pseudomonas aeruginosa crystallized in GTP presence
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-04-22 |
Detector | Bruker Platinum 135 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 61.562, 72.863, 107.275 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 21.598 - 2.140 |
R-factor | 0.1737 |
Rwork | 0.171 |
R-free | 0.21890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1u1t |
RMSD bond length | 0.008 |
RMSD bond angle | 1.150 |
Data reduction software | PROTEUM PLUS (PLUS) |
Data scaling software | PROTEUM PLUS (PLUS) |
Phasing software | PHENIX (1.8.1_1168) |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 21.598 | 2.260 |
High resolution limit [Å] | 2.140 | 2.140 |
Rmerge | 0.079 | 0.385 |
Number of reflections | 25623 | |
<I/σ(I)> | 16.23 | 5.53 |
Completeness [%] | 94.1 | 79.62 |
Redundancy | 6.1 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 200 MM ammonium sulphate, 200 mm NaCl, 50 MM TRIS-HCL, PH 8.0, 4.75 mM GTP, VAPOR DIFFUSION, HANGING DROP, temperature 295K |