4J6Y

Hfq from Pseudomonas aeruginosa crystallized in GTP presence

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	100
Detector technology	CCD
Collection date	2012-04-22
Detector	Bruker Platinum 135
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	61.562, 72.863, 107.275
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.598 - 2.140
R-factor	0.1737
Rwork	0.171
R-free	0.21890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1u1t
RMSD bond length	0.008
RMSD bond angle	1.150
Data reduction software	PROTEUM PLUS (PLUS)
Data scaling software	PROTEUM PLUS (PLUS)
Phasing software	PHENIX (1.8.1_1168)
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	21.598	2.260
High resolution limit [Å]	2.140	2.140
Rmerge	0.079	0.385
Number of reflections	25623
<I/σ(I)>	16.23	5.53
Completeness [%]	94.1	79.62
Redundancy	6.1	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	295	200 MM ammonium sulphate, 200 mm NaCl, 50 MM TRIS-HCL, PH 8.0, 4.75 mM GTP, VAPOR DIFFUSION, HANGING DROP, temperature 295K