4J6V
Crystal Structure of Tyrosinase from Bacillus megaterium N205D mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.900, 78.330, 82.160 |
| Unit cell angles | 90.00, 105.50, 90.00 |
Refinement procedure
| Resolution | 39.392 - 1.900 |
| R-factor | 0.194 |
| Rwork | 0.193 |
| R-free | 0.21010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.396 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | PHASER (2.3.0) |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 79.172 | 43.841 | 2.000 |
| High resolution limit [Å] | 1.900 | 6.010 | 1.900 |
| Rmerge | 0.069 | 0.063 | 0.251 |
| Total number of observations | 3775 | 26596 | |
| Number of reflections | 51526 | ||
| <I/σ(I)> | 11.7 | 9 | 1.6 |
| Completeness [%] | 97.6 | 71.1 | 98.1 |
| Redundancy | 3.5 | 3.1 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1 M sodium cacodylate, pH 6.5, 18% PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
