4J6U
Crystal Structure of Tyrosinase from Bacillus megaterium N205A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9393 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.080, 78.770, 85.990 |
| Unit cell angles | 90.00, 104.63, 90.00 |
Refinement procedure
| Resolution | 28.601 - 2.500 |
| R-factor | 0.2092 |
| Rwork | 0.207 |
| R-free | 0.24070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3nm8 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.555 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.16) |
| Phasing software | PHASER (2.1.4) |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 83.202 | 28.601 | 2.510 |
| High resolution limit [Å] | 2.385 | 7.540 | 2.385 |
| Rmerge | 0.099 | 0.050 | 0.191 |
| Total number of observations | 2513 | 11154 | |
| Number of reflections | 24549 | ||
| <I/σ(I)> | 6.4 | 11.8 | 3.3 |
| Completeness [%] | 98.9 | 89 | 98 |
| Redundancy | 3.4 | 3.4 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1 M sodium cacodylate, pH 6.5, 18% PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
