4J6S
14-3-3gamma complexed with the N-terminal sequence of tyrosine hydroxylase (residues 1-43)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-15 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0331 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 84.190, 115.120, 136.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.900 - 3.080 |
| R-factor | 0.21223 |
| Rwork | 0.210 |
| R-free | 0.25377 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a4o |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.192 |
| Data reduction software | XDS (package) |
| Data scaling software | XDS (package) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.160 |
| High resolution limit [Å] | 3.080 | 3.080 |
| Rmerge | 0.111 | 0.492 |
| Number of reflections | 111792 | |
| <I/σ(I)> | 11.6 | 3.29 |
| Completeness [%] | 87.1 | 90 |
| Redundancy | 5.1 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.1 | 283 | 30% PEG 2000 MME, 0.1M Potassium Thiocyanate, pH 7.1, VAPOR DIFFUSION, temperature 283.0K |
