4J5V

Crystal structure of p19 in complex with double-helical RNA 19mer p(CAG)3C(CCG)3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-10-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9724
Spacegroup name	H 3 2
Unit cell lengths	90.219, 90.219, 148.039
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 - 2.150
R-factor	0.17369
Rwork	0.172
R-free	0.21401
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.024
RMSD bond angle	2.114
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	REFMAC
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.190
High resolution limit [Å]	2.150	5.820	2.150
Rmerge	0.100	0.056	0.134
Number of reflections	12580
<I/σ(I)>	19.5
Completeness [%]	98.2	98.9	95.9
Redundancy	3.1	4.2	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	1.7 mM ammonium sulfate, 0.1 M MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K