4J5T
Crystal structure of Processing alpha-Glucosidase I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-18 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.997 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 94.800, 101.800, 103.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.000 - 2.040 |
| R-factor | 0.22626 |
| Rwork | 0.226 |
| R-free | 0.23467 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.890 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.000 | 2.110 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Rmerge | 0.089 | 0.477 |
| Number of reflections | 63223 | |
| <I/σ(I)> | 9.3 | 3.4 |
| Completeness [%] | 98.7 | 98.9 |
| Redundancy | 6.3 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 4mg/mL protein in 20mM potassium phosphate pH 6.8 100mM NaCl; Crystallized in 1:1 drops with reservoir solution (15% PEG 4000 0.1M MES pH 6.5, 0.1M sodium phosphate, pH 6.5, 0.1M potassium phosphate pH 6.5, 0.6M NaCl) , VAPOR DIFFUSION, SITTING DROP, temperature 298K |
