4J5T
Crystal structure of Processing alpha-Glucosidase I
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-18 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.997 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 94.800, 101.800, 103.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.000 - 2.040 |
R-factor | 0.22626 |
Rwork | 0.226 |
R-free | 0.23467 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 0.890 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.000 | 2.110 |
High resolution limit [Å] | 2.040 | 2.040 |
Rmerge | 0.089 | 0.477 |
Number of reflections | 63223 | |
<I/σ(I)> | 9.3 | 3.4 |
Completeness [%] | 98.7 | 98.9 |
Redundancy | 6.3 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 4mg/mL protein in 20mM potassium phosphate pH 6.8 100mM NaCl; Crystallized in 1:1 drops with reservoir solution (15% PEG 4000 0.1M MES pH 6.5, 0.1M sodium phosphate, pH 6.5, 0.1M potassium phosphate pH 6.5, 0.6M NaCl) , VAPOR DIFFUSION, SITTING DROP, temperature 298K |