4J5O

Room temperature crystal structure of a RNA binding motif protein 39 (Rbm39) from Mus musculus at 1.11 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	296
Detector technology	PIXEL
Collection date	2012-05-30
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 21 21 21
Unit cell lengths	44.617, 52.829, 85.652
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.551 - 1.110
R-factor	0.1134
Rwork	0.113
R-free	0.12890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3s6e
RMSD bond length	0.012
RMSD bond angle	1.491
Data reduction software	XDS
Data scaling software	XSCALE (March 15, 2012)
Phasing software	REFMAC
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.551	28.550	1.140
High resolution limit [Å]	1.110	4.970	1.110
Rmerge	0.055	0.033	1.016
Number of reflections	75696	917	3368
<I/σ(I)>	18.4	67.3	2.2
Completeness [%]	94.2
Redundancy	9.7	10.4	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	277	20.00% polyethylene glycol 6000, 0.1M sodium citrate pH 5.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K