4J5L
Structure of the Cargo Binding Domain from Human Myosin Va
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-24 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.4586 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.799, 79.494, 94.139 |
| Unit cell angles | 90.00, 105.77, 90.00 |
Refinement procedure
| Resolution | 39.356 - 2.200 |
| R-factor | 0.2081 |
| Rwork | 0.205 |
| R-free | 0.26410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.598 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.356 | 2.251 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.081 | 0.272 |
| Number of reflections | 41574 | |
| <I/σ(I)> | 12.1 | 4.1 |
| Completeness [%] | 96.0 | 83.2 |
| Redundancy | 3.3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 0.1M bis-tris, 22% PEG 3350, 0.2M ammonium sulfate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
