4J5K
Crystal structure analysis of Streptomyces aureofaciens ribonuclease Sa Y51F mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X31 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-07-09 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.303, 63.813, 77.624 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.590 - 1.230 |
| R-factor | 0.10569 |
| Rwork | 0.104 |
| R-free | 0.13568 |
| Starting model (for MR) | 1t2h |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.108 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | model (structure was used without molecular replacement) |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.240 |
| High resolution limit [Å] | 1.230 | 1.230 |
| Rmerge | 0.037 | 0.139 |
| Number of reflections | 54845 | |
| <I/σ(I)> | 29.7 | 4.9 |
| Completeness [%] | 97.8 | 82.6 |
| Redundancy | 2.3 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | Ammonium sulfate 0.9 M, phosphate buffer 0.1 M , pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
