4J4Y
Triple mutant GraVN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-26 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 |
| Unit cell lengths | 54.160, 76.798, 77.156 |
| Unit cell angles | 82.79, 69.51, 69.36 |
Refinement procedure
| Resolution | 28.810 - 2.450 |
| R-factor | 0.21412 |
| Rwork | 0.210 |
| R-free | 0.29102 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4j4r |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.639 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.830 | 2.590 |
| High resolution limit [Å] | 2.450 | 2.500 |
| Number of reflections | 35932 | |
| Completeness [%] | 95.2 | 91.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 0.1M Ca(OAc)2, 12%(w/v) PEG3350, 0.04M citric acid, 0.06M Bis-tris, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
