4J4O
Crystal structure of FK506 binding domain of plasmodium VIVAX FKBP35 in complex with D44
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2011-09-26 |
Detector | RIGAKU RAXIS IV++ |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 68.259, 68.259, 74.116 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 1.730 |
R-factor | 0.179 |
Rwork | 0.177 |
R-free | 0.21500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ihz |
RMSD bond length | 0.014 |
RMSD bond angle | 1.563 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.500 | 1.790 |
High resolution limit [Å] | 1.730 | 1.730 |
Rmerge | 0.049 | 0.187 |
Number of reflections | 21268 | |
<I/σ(I)> | 22.4 | 6.9 |
Completeness [%] | 99.8 | 99.7 |
Redundancy | 10.32 | 7.93 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 291 | 3.0M AMMONIUM SULPHATE, 0.1M BICINE, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |