4J4O
Crystal structure of FK506 binding domain of plasmodium VIVAX FKBP35 in complex with D44
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-09-26 |
| Detector | RIGAKU RAXIS IV++ |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 68.259, 68.259, 74.116 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.730 |
| R-factor | 0.179 |
| Rwork | 0.177 |
| R-free | 0.21500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ihz |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.563 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.500 | 1.790 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Rmerge | 0.049 | 0.187 |
| Number of reflections | 21268 | |
| <I/σ(I)> | 22.4 | 6.9 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 10.32 | 7.93 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 291 | 3.0M AMMONIUM SULPHATE, 0.1M BICINE, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
