4J3T
Crystal structure of barley Limit dextrinase co-crystallized with 25mM maltotetraose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-23 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.04 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 175.090, 86.230, 61.510 |
| Unit cell angles | 90.00, 95.58, 90.00 |
Refinement procedure
| Resolution | 29.700 - 1.600 |
| R-factor | 0.1347 |
| Rwork | 0.133 |
| R-free | 0.17690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2y4s |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.282 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.700 | |
| High resolution limit [Å] | 1.600 | 4.740 | 1.600 |
| Rmerge | 0.063 | 0.031 | 0.397 |
| Rmeas | 0.037 | 0.467 | |
| Number of reflections | 119393 | 4630 | 18764 |
| <I/σ(I)> | 14.45 | 34.27 | 3.43 |
| Completeness [%] | 99.4 | 98 | 97.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 20% PEG 3350, 0.3M NaI, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
