4J3O
Crystal structure of the FimD usher traversed by the pilus tip complex assembly composed of FimC:FimF:FimG:FimH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-12-06 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9795 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 122.360, 122.360, 328.460 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 83.668 - 3.800 |
R-factor | 0.2503 |
Rwork | 0.248 |
R-free | 0.29860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3BWU for FimC:FimF PDB 3RFZ for FimD PDB 3JWN for FimG:FimH |
RMSD bond length | 0.002 |
RMSD bond angle | 0.455 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 83.670 | 3.900 |
High resolution limit [Å] | 3.800 | 3.800 |
Number of reflections | 25474 | |
Completeness [%] | 99.8 | 99.96 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 1.6-2.0M sodium formate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |