4J3O

Crystal structure of the FimD usher traversed by the pilus tip complex assembly composed of FimC:FimF:FimG:FimH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	CCD
Collection date	2010-12-06
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	P 42 21 2
Unit cell lengths	122.360, 122.360, 328.460
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	83.668 - 3.800
R-factor	0.2503
Rwork	0.248
R-free	0.29860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3BWU for FimC:FimF PDB 3RFZ for FimD PDB 3JWN for FimG:FimH
RMSD bond length	0.002
RMSD bond angle	0.455
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	83.670	3.900
High resolution limit [Å]	3.800	3.800
Number of reflections	25474
Completeness [%]	99.8	99.96

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	1.6-2.0M sodium formate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K