4J3H

Ring cycle for dilating and constricting the nuclear pore: structure of a Nup54 homo-tetramer.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-10-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 61 2 2
Unit cell lengths	41.320, 41.320, 198.410
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.661 - 1.500
R-factor	0.1949
Rwork	0.192
R-free	0.22270
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.240
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.7.2_869))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	1.700
High resolution limit [Å]	1.500	1.500
Number of reflections	30293
<I/σ(I)>	38.4	13.3
Completeness [%]	99.7	99.4
Redundancy	24	22

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.3	293	22-26% PEG 400, 0.1 M Na Hepes buffer pH 7.1 - 8.1, 0.2 M CaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 293K