4J3H
Ring cycle for dilating and constricting the nuclear pore: structure of a Nup54 homo-tetramer.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 41.320, 41.320, 198.410 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.661 - 1.500 |
| R-factor | 0.1949 |
| Rwork | 0.192 |
| R-free | 0.22270 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.240 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 1.700 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 30293 | |
| <I/σ(I)> | 38.4 | 13.3 |
| Completeness [%] | 99.7 | 99.4 |
| Redundancy | 24 | 22 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 293 | 22-26% PEG 400, 0.1 M Na Hepes buffer pH 7.1 - 8.1, 0.2 M CaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
