4J3C
Crystal structure of 16S ribosomal RNA methyltransferase RsmE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-01-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9733 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 96.085, 152.082, 38.995 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.24838 |
| Rwork | 0.246 |
| R-free | 0.28690 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.496 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.060 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.070 | 0.716 |
| Number of reflections | 39489 | |
| <I/σ(I)> | 11.2 | |
| Completeness [%] | 99.7 | 97.8 |
| Redundancy | 10.6 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 10% PEG3350, 0.1M HEPES, 0.2M L-Proline, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
