4J38
Structure of Borrelia burgdorferi Outer surface protein E in complex with Factor H domains 19-20
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97372 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 86.405, 86.405, 107.784 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.509 - 2.830 |
| R-factor | 0.1964 |
| Rwork | 0.193 |
| R-free | 0.25480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g7i |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.372 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | Rosetta |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.830 | 2.830 |
| Rmerge | 0.170 | 1.098 |
| Number of reflections | 12063 | |
| <I/σ(I)> | 9.88 | 2.2 |
| Completeness [%] | 99.2 | 95.2 |
| Redundancy | 7.05 | 7.08 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 2M Ammonium sulfate, 0.1M Citric acid, 0.2M Sodium chloride, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
