4J30
Structure of the effector - immunity system Tae4 / Tai4 from Salmonella typhimurium, selenomethionine variant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-08-18 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.97933 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 63.380, 63.380, 364.990 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.800 - 2.300 |
R-factor | 0.2001 |
Rwork | 0.198 |
R-free | 0.23138 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.120 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELXD |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.400 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.154 | 0.587 |
Number of reflections | 36560 | |
<I/σ(I)> | 24 | 7.1 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 42 | 43 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 200 mM tri-sodium citrate pH 6.0, 30 % (v/v) 2-ethoxyethanol , VAPOR DIFFUSION, HANGING DROP, temperature 293K |