4J2R

Middle domain of influenza A virus RNA-dependent polymerase PB2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2012-12-06
Detector	ADSC QUANTUM 210r
Wavelength(s)	1.0
Spacegroup name	I 21 21 21
Unit cell lengths	75.969, 103.511, 112.526
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.880 - 2.420
R-factor	0.21828
Rwork	0.215
R-free	0.27110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vqz
RMSD bond length	0.015
RMSD bond angle	1.787
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP (11.0)
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.420	2.470
High resolution limit [Å]	2.420	2.420
Rmerge	0.045	0.330
Number of reflections	16108
<I/σ(I)>	45	45.1
Completeness [%]	93.5	98.9
Redundancy	7.1	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.9	277	0.1M NaH2PO4, 0.1M KH2PO4, 0.1M MES (pH 5.9), 0.8M NaCl, 4% (v/v) 1-propanol, VAPOR DIFFUSION, HANGING DROP, temperature 277K