4J2Q
Crystal structure of C-terminally truncated arrestin reveals mechanism of arrestin activation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9395 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 102.494, 102.494, 464.396 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.650 - 3.000 |
| R-factor | 0.24942 |
| Rwork | 0.248 |
| R-free | 0.27945 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cf1 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.231 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.650 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.106 | 0.502 |
| Number of reflections | 28594 | |
| <I/σ(I)> | 10.9 | 2.4 |
| Completeness [%] | 95.8 | 96 |
| Redundancy | 3.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 30% polyethylene glycol 200, 10 mM HEPES, 100 mM lithium sulfate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
