4J2P
Crystal structure of LuxF from Photobacterium leiognathi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-18 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9794 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 93.180, 93.180, 115.430 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.200 - 1.850 |
| R-factor | 0.1723 |
| Rwork | 0.171 |
| R-free | 0.20400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nfp |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.007 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.5.2) |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 39.200 | 39.200 | 1.950 |
| High resolution limit [Å] | 1.850 | 30.000 | 1.850 |
| Rmerge | 0.044 | 0.012 | 0.660 |
| Number of reflections | 22033 | 5 | 3164 |
| <I/σ(I)> | 41 | 56.7 | 3.14 |
| Completeness [%] | 99.9 | 55.6 | 99.7 |
| Redundancy | 13.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | drops under oil | 7 | 293 | 0.15M malic acid, 20% w/v polyethylene glycol 3,350, pH 7.0, drops under oil, temperature 293K |
