4J2N

Crystal Structure of mycobacteriophage Pukovnik Xis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E DW
Temperature [K]	100
Detector technology	CCD
Collection date	2009-07-15
Detector	RIGAKU SATURN 944
Wavelength(s)	1.5418
Spacegroup name	C 2 2 21
Unit cell lengths	89.423, 129.985, 92.346
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.921 - 2.348
R-factor	0.2294
Rwork	0.226
R-free	0.26700
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.984
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX (1.6.1_357)
Refinement software	PHENIX (1.6.1_357)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	90.000	2.390
High resolution limit [Å]	2.350	2.350
Number of reflections	22815
<I/σ(I)>		2.2
Completeness [%]	99.8	100
Redundancy		4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	300	ammonium sulfate, PEG 3350, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 300K
1	VAPOR DIFFUSION, SITTING DROP	8	300	ammonium sulfate, PEG 3350, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 300K