4J2F
Crystal structure of a glutathione transferase family member from Ricinus communis, target EFI-501866
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.075 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 92.556, 112.438, 54.408 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.280 - 1.900 |
| R-factor | 0.20556 |
| Rwork | 0.203 |
| R-free | 0.24810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gwc |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.310 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.078 | 0.789 |
| Number of reflections | 22129 | |
| <I/σ(I)> | 28 | |
| Completeness [%] | 97.2 | 95.6 |
| Redundancy | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.2M lithium sulfate, 0.1M Tris-HCl (PH 8.5), 25% PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
