4J2C
GARP-SNARE Interaction
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.636, 45.660, 58.338 |
| Unit cell angles | 90.00, 90.04, 90.00 |
Refinement procedure
| Resolution | 41.636 - 1.801 |
| R-factor | 0.1549 |
| Rwork | 0.153 |
| R-free | 0.19460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.827 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.6.1_357) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 3.880 | 1.800 |
| Rmerge | 0.048 | 0.027 | 0.358 |
| Number of reflections | 19877 | ||
| <I/σ(I)> | 12.8 | ||
| Completeness [%] | 97.2 | 99 | 91 |
| Redundancy | 2.7 | 2.7 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 291 | 18% PEG 3350, 100mM Ammonium Dihydrogen Phosphate, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
