4J1M
Crystal structure of Staphylococcal nuclease variant Delta+PHS R105E at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-08 |
| Detector | APEX II CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 30.993, 60.701, 37.909 |
| Unit cell angles | 90.00, 94.85, 90.00 |
Refinement procedure
| Resolution | 30.880 - 1.650 |
| R-factor | 0.1811 |
| Rwork | 0.179 |
| R-free | 0.21420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bdc |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.763 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | PHASER (2.3.0) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.750 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.026 | 0.348 |
| Number of reflections | 16916 | |
| <I/σ(I)> | 24.69 | 2.54 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.63 | 5.04 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 27% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
