4J1J
Leanyer orthobunyavirus nucleoprotein-ssDNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 1 |
| Unit cell lengths | 50.403, 73.199, 77.905 |
| Unit cell angles | 70.89, 82.54, 73.73 |
Refinement procedure
| Resolution | 43.330 - 2.650 |
| R-factor | 0.2236 |
| Rwork | 0.223 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4j1g |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.282 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.330 | 2.780 |
| High resolution limit [Å] | 2.650 | 2.680 |
| Number of reflections | 27429 | |
| Completeness [%] | 94.7 | 84 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 0.2 M ammonium sulfate, 0.1 M Tris-HCl (pH 7.5) and 20% w/v PEG5000 MME, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
